The regular players from Seigaku participates in an exhibition game aboard a cruise ship. However, they discover that the event is being run by a corrupt millionaire heavily involved in illegal gambling and betting, who demands that they lose the match on purpose. To add to the confusion, one of the players on the rival team introduces himself as Ryoma's brother.
Unfortunately the movie The Prince of Tennis: Two Samurais, The First Game is not yet available on HBO Max.
| Visual Effects | Minoru Ueta | Key Animation |
| Writing | Atsushi Maekawa | Scenario Writer |
| Visual Effects | Atsushi Takeuchi | Key Animation |
| Visual Effects | Eri Baba | Key Animation |
| Visual Effects | Ken Ootsuka | Key Animation |
| Visual Effects | Kazunori Akiyama | Key Animation |
| Sound | Cher Watanabe | Music |
| Editing | Takamitsu Inoue | Assistant Editor |
| Visual Effects | Shinichi Iimura | Key Animation |
| Visual Effects | Shinichi Suzuki | Key Animation |
| Visual Effects | Nobuo Hashimoto | Key Animation |
| Visual Effects | Shinichiro Minami | Key Animation |
| Visual Effects | Satoru Utsunomiya | Key Animation |
| Visual Effects | Jun Yoshida | Key Animation |
| Visual Effects | Keigo Sasaki | Key Animation |
| Visual Effects | Chiho Mifune | Key Animation |
| Visual Effects | Takeshi Honda | Key Animation |
| Visual Effects | Keiko Shimizu | Key Animation |
| Visual Effects | Shinya Ohira | Key Animation |
| Visual Effects | Futoshi Higashide | Key Animation |
| Visual Effects | Haruko Iizuka | Key Animation |
| Visual Effects | Hiroyuki Okiura | Key Animation |
| Visual Effects | Kyoji Asano | Key Animation |
| Visual Effects | Yasunori Miyazawa | Key Animation |
| Visual Effects | Tadashi Kubo | Key Animation |
| Visual Effects | Etsuko Tomita | Key Animation |
| Visual Effects | Hitoshi Haga | Key Animation |
| Visual Effects | Masahiko Harano | 3D Artist |
| Art | Takayuki Hamana | Storyboard Artist |
| Writing | Takeshi Konomi | Original Story |
| Directing | Takayuki Hamana | Director |
| Art | Shuichi Hirata | Art Direction |
| Production | Yoko Matsushita | Executive Producer |
| Visual Effects | Akiharu Ishii | Character Designer |
| Writing | Hiroshi Yamazaki | Storyboard |
| Writing | Kazunobu Fuseki | Storyboard |
| Production | Shouko Nogami | Casting |
| Camera | Hisashi Ezura | Director of Photography |
| Production | Tsuyoshi Yoshida | Executive Producer |
| Crew | Masahiro Murakami | Special Effects |
| Visual Effects | Minoru Ueta | Animation Director |
| Visual Effects | Miyako Yatsu | Animation Director |
| Production | Tomoko Takahashi | Producer |
| Visual Effects | Sayuri Yoshida | Color Designer |
| Editing | Junichi Uematsu | Editor |
| Production | Susumu Matsuyama | Producer |
| Sound | Yasufumi Yoda | Sound Effects |
| Production | Kazuhiko Torishima | Executive Producer |
| Production | Keisuke Iwata | Executive Producer |
| Production | Tetsu Kawaguchi | Producer |
| Sound | Takuya Hiramitsu | Sound Director |
| Visual Effects | Eiji Mizutani | 3D Animator |
