| Season 1 | Episode 1 | Rescue the Red-Eyed Tree Frogs! | 2005-09-06 | 30 min |
| Season 1 | Episode 2 | Diego Saves Mommy and Baby Sloth | 2005-09-07 | 30 min |
| Season 1 | Episode 3 | Chinta the Baby Chinchilla | 2005-09-08 | 30 min |
| Season 1 | Episode 4 | Diego Saves Baby Humpback Whale | 2005-09-09 | 30 min |
| Season 1 | Episode 5 | Journey to Jaguar Mountain | 2005-09-12 | 30 min |
| Season 1 | Episode 6 | Three Little Condors | 2005-09-13 | 30 min |
| Season 1 | Episode 7 | Pepito's Penguin School | 2005-09-14 | 30 min |
| Season 1 | Episode 8 | Armadillo's Rainforest Race | 2005-09-15 | 30 min |
| Season 1 | Episode 9 | The Mommy Macaw | 2005-09-16 | 30 min |
| Season 1 | Episode 10 | Linda the Llama Saves Carnival | 2005-10-10 | 30 min |
| Season 1 | Episode 11 | A Booboo on the Pygmy Marmoset | 2006-01-23 | 30 min |
| Season 1 | Episode 12 | A Blue Morpho Butterfly is Born | 2006-01-25 | 30 min |
| Season 1 | Episode 13 | Cool Water for Ana the Anaconda | 2006-01-26 | 30 min |
| Season 1 | Episode 14 | Linda the Librarian | 2006-03-20 | 30 min |
| Season 1 | Episode 15 | Chito and Rita the Spectacled Bears | 2006-02-20 | 30 min |
| Season 1 | Episode 16 | The Great Dinosaur Adventure (1) | 2006-03-07 | 30 min |
| Season 1 | Episode 17 | The Great Dinosaur Adventure (2) | 2006-03-09 | 30 min |
| Season 1 | Episode 18 | Diego's Wolf Pup Rescue | 2006-03-19 | 30 min |
| Season 1 | Episode 19 | Baby Jaguar to the Rescue | 2006-03-26 | 30 min |
| Season 1 | Episode 20 | Save the Sea Turtles | 2006-07-10 | 30 min |
| Season 2 | Episode 1 | Diego Saves the River Dolphin | 2006-10-02 | 30 min |
| Season 2 | Episode 2 | Diego and Baby Humpback to the Rescue | 2006-10-03 | 30 min |
| Season 2 | Episode 3 | Little Kinkajou is in Beehive Trouble | 2006-10-30 | 30 min |
| Season 2 | Episode 4 | Mackey the Macaroni Penguin | 2006-10-31 | 30 min |
| Season 2 | Episode 5 | The Iguana Sing Along | 2006-11-19 | 30 min |
| Season 2 | Episode 6 | Panchita Prairie Dog Finds a New Prairie | 2006-12-03 | 30 min |
| Season 2 | Episode 7 | Diego Saves Christmas | 2006-12-08 | 30 min |
| Season 2 | Episode 8 | Baby Jaguar's Lost Growl | 2007-01-15 | 30 min |
| Season 2 | Episode 9 | Diego the Hero | 2007-02-05 | 30 min |
| Season 2 | Episode 10 | Sammy's Valentine | 2007-02-14 | 30 min |
| Season 2 | Episode 11 | The Great Roadrunner Race | 2007-03-26 | 30 min |
| Season 2 | Episode 12 | The Tapir's Trip Home | 2007-03-06 | 30 min |
| Season 2 | Episode 13 | Jorge the Little Hawk Learns to Migrate | 2007-03-07 | 30 min |
| Season 2 | Episode 14 | Giant Octopus to the Rescue | 2007-03-08 | 30 min |
| Season 2 | Episode 15 | An Underwater Mystery | 2007-06-26 | 30 min |
| Season 2 | Episode 16 | Diego's African Safari (1) | 2007-10-08 | 30 min |
| Season 2 | Episode 17 | Diego's African Safari (2) | 2007-10-15 | 30 min |
| Season 2 | Episode 18 | Diego and Alicia Save the Otters | 2007-10-16 | 30 min |
| Season 2 | Episode 19 | Alicia Saves the Crocodile | 2007-11-24 | 30 min |
| Season 2 | Episode 20 | Rhea is an Animal Rescuer | 2007-12-19 | 30 min |
| Season 3 | Episode 1 | Green Iguana Helps Abuelito Plant a New Strawberry Farm! | 2008-02-05 | 30 min |
| Season 3 | Episode 2 | Kicho's Magic Flute! | 2008-03-02 | 30 min |
| Season 3 | Episode 3 | Rainforest Rhapsody | 2008-03-03 | 30 min |
| Season 3 | Episode 4 | Gorilla Fun | 2008-03-04 | 30 min |
| Season 3 | Episode 5 | Tuga Helps the Moon | 2008-03-18 | 30 min |
| Season 3 | Episode 6 | Whistling Willie Finds a Buddy | 2008-03-21 | 30 min |
| Season 3 | Episode 7 | Super Flying Squirrel to the Rescue! | 2008-04-28 | 30 min |
| Season 3 | Episode 8 | Diego and Porcupine Save the Pinata! | 2008-05-02 | 30 min |
| Season 3 | Episode 9 | The Bobo's Mother's Day! | 2009-05-09 | 30 min |
| Season 3 | Episode 10 | It's a Bug's World! | 2008-05-12 | 30 min |
| Season 3 | Episode 11 | Diego and Dora Help Baby Monarch Get to the Festival | 2008-05-31 | 30 min |
| Season 3 | Episode 12 | Save the Giant Tortoises | 2008-06-03 | 30 min |
| Season 3 | Episode 13 | Alicia and Whitetail to the Rescue | 2008-06-06 | 30 min |
| Season 3 | Episode 14 | Manatee's Mermaid Rescue! | 2008-06-20 | 30 min |
| Season 3 | Episode 15 | Freddie the Fruit Bat Saves Halloween! | 2008-10-27 | 30 min |
| Season 3 | Episode 16 | Egyptian Camel Adventure | 2008-11-21 | 30 min |
| Season 3 | Episode 17 | A New Flamingo Mami | 2009-03-05 | 30 min |
| Season 3 | Episode 18 | The Great Polar Bear Rescue | 2009-09-18 | 30 min |
| Season 4 | Episode 1 | Diego Reunites Hippo & Oxpecker | 2009-09-28 | 30 min |
| Season 4 | Episode 2 | Diego's Orangutan Rescue | 2009-09-29 | 30 min |
| Season 4 | Episode 3 | Where is Okapi's Brother? | 2009-09-30 | 30 min |
| Season 4 | Episode 4 | Puffin Fish Adventure | 2009-10-01 | 30 min |
| Season 4 | Episode 5 | Diego's Ringed Seal Rescue | 2010-02-19 | 30 min |
| Season 4 | Episode 6 | All Aboard the Giant Panda Express! | 2010-03-15 | 30 min |
| Season 4 | Episode 7 | Bengal Tiger Makes a Wish | 2010-03-16 | 30 min |
| Season 4 | Episode 8 | Leaping Lemurs! | 2010-03-17 | 30 min |
| Season 4 | Episode 9 | Diego Saves the Beavers | 2010-03-18 | 30 min |
| Season 4 | Episode 10 | Ocean Animal Rescuer | 2010-04-22 | 30 min |
| Season 4 | Episode 11 | Welcome Home, Lion Cub | 2010-06-18 | 30 min |
| Season 4 | Episode 12 | Diego's International Rescue League | 2010-09-24 | 30 min |
| Season 5 | Episode 1 | Diego Rescues Prince Vicuna | 2010-11-05 | 30 min |
| Season 5 | Episode 2 | Koala's Birthday Hug | 2011-02-07 | 30 min |
| Season 5 | Episode 3 | To Babysit a Bobo | 2011-02-08 | 30 min |
| Season 5 | Episode 4 | Pampas Cat & Friends Help the Rescue Center | 2011-02-09 | 30 min |
| Season 5 | Episode 5 | Cotton-Top Tamarin Cave Rescue | 2011-02-10 | 30 min |
| Season 5 | Episode 6 | Fiercest Animals! | 2011-07-16 | 30 min |
